1. Microgravity promotes osteoclast activity in medaka fish reared at the ...
21 sep 2015 · The bone mineral density (BMD) of astronauts decreases specifically in the weight-bearing sites during spaceflight.
The bone mineral density (BMD) of astronauts decreases specifically in the weight-bearing sites during spaceflight. It seems that osteoclasts would be affected by a change in gravity; however, the molecular mechanism involved remains unclear. Here, ...
2. 3D Structural Fluctuation of IgG1 Antibody Revealed by Individual ...
5 mei 2015 · Akiko Ishii-Watabe. Scientific Reports (2023). Effective clearance of rituximab-resistant tumor cells by breaking the mirror-symmetry of ...
Commonly used methods for determining protein structure, including X-ray crystallography and single-particle reconstruction, often provide a single and unique three-dimensional (3D) structure. However, in these methods, the protein dynamics and flexibility/fluctuation remain mostly unknown. Here, we utilized advances in electron tomography (ET) to study the antibody flexibility and fluctuation through structural determination of individual antibody particles rather than averaging multiple antibody particles together. Through individual-particle electron tomography (IPET) 3D reconstruction from negatively-stained ET images, we obtained 120 ab-initio 3D density maps at an intermediate resolution (~1–3 nm) from 120 individual IgG1 antibody particles. Using these maps as a constraint, we derived 120 conformations of the antibody via structural flexible docking of the crystal structure to these maps by targeted molecular dynamics simulations. Statistical analysis of the various conformations disclosed the antibody 3D conformational flexibility through the distribution of its domain distances and orientations. This blueprint approach, if extended to other flexible proteins, may serve as a useful methodology towards understanding protein dynamics and functions.
3. A Novel Diabetes Mellitus Mouse Model, MAFA-Deficient and Beta ...
Many models of diabetic nephropathy have been reported. However, it is rare that the characteristic findings of severe human diabetic nephropathy, ...
Many models of diabetic nephropathy have been reported. However, it is rare that the characteristic findings of severe human diabetic nephropathy, suc …
4. Novel banana lectin CAR-T cells to target pancreatic tumors and tumor ...
18 jan 2023 · Challice Bonifant Department of Oncology, Sidney Kimmel ... Akiko Ishii-Watabe, Pharmacol Res Pers, 2016. Powered by · Privacy ...
Background Cell therapies for solid tumors are thwarted by the hostile tumor microenvironment (TME) and by heterogeneous expression of tumor target antigens. We address both limitations with a novel class of chimeric antigen receptors based on plant lectins, which recognize the aberrant sugar residues that are a ‘hallmark’ of both malignant and associated stromal cells. We have expressed in T cells a modified lectin from banana, H84T BanLec, attached to a chimeric antigen receptor (H84T-CAR) that recognizes high-mannose (asparagine residue with five to nine mannoses). Here, we tested the efficacy of our novel H84T CAR in models of pancreatic ductal adenocarcinoma (PDAC), intractable tumors with aberrant glycosylation and characterized by desmoplastic stroma largely contributed by pancreatic stellate cells (PSCs). Methods We transduced human T cells with a second-generation retroviral construct expressing the H84T BanLec chimeric receptor, measured T-cell expansion, characterized T-cell phenotype, and tested their efficacy against PDAC tumor cells lines by flow cytometry quantification. In three-dimensional (3D) spheroid models, we measured H84T CAR T-cell disruption of PSC architecture, and T-cell infiltration by live imaging. We tested the activity of H84T CAR T cells against tumor xenografts derived from three PDAC cell lines. Antitumor activity was quantified by caliper measurement and bioluminescence signal and used anti-human vimentin to measure residual PSCs. Results...
5. [PDF] Biological Reference Data on CD(SD) IGS Rats-2002/2003 CD(SD)IGS ...
This is the fifth publication of Biological Reference Data on CD(SD)IGS Rats-2002/2003 and is the last volume to be published by CD(SD)IGS Study Group in ...
6. Impact of feline AIM on the susceptibility of cats to renal disease
Chawla, L. S. & Kimmel, P. L. Acute kidney injury and chronic kidney ... Akiko Takahata, Satoko Arai, Emiri Hiramoto, Kento Kitada, Rina Kato, Yuko ...
AbstractRenal failure is one of the most important social problems for its incurability and high costs for patients’ health care. Through clarification of the underlying mechanism for the high susceptibility of cats to renal disease, we here demonstrates that the effective dissociation of serum AIM protein from IgM is necessary for the recovery from acute kidney injury (AKI). In cats, the AIM-IgM binding affinity is 1000-fold higher than that in mice, which is caused by the unique positively-charged amino-…
See AlsoN-Deklination (Complete Guide)
7. Ostrava, CZE - 2024 - Akiko/Ishii - JPN - Women's Teams - Volleyball World
Bevat niet: kimmel | Resultaten tonen met:kimmel
Get Elite16 - Ostrava, CZE - 2024 - Beach Pro Tour 2024 - Akiko/Ishii - Japan - Women's team list
8. Researcher Directory - Sekiguchi Kiyotoshi - 大阪大学 研究者総覧
... Ishii,Hidetoshi Sakurai,Nobuharu Suzuki,Yo Mabuchi,Ichiro Sekiya,Kiyotoshi ... Kimmel,Kiyotoshi Sekiguchi,Raimund Wagener,Heinz Schwarz,Phillip W.
大阪大学研究者総覧
9. Program | The 50th Annual Meeting of the Japanese Society for Immunology
Akiko Iwasaki（Department of Immunobiology, Yale University School of ... Ishii from Institute of Medical Science, The University of Tokyo. Each talk ...
At a Glance
10. [DOC] please click here - NCI at Frederick - National Cancer Institute
KIMMEL, PAUL, NIDDK. KLARMANN, KIM, BTB/DCTD. KLAUZINSKA, MARGARET, DCB. KLICKA ... AKIKO, DANA FARBER/BOSTON CHILDRENS CANCER AND BLOOD DISORDERS CENTER. SHOMALI ...
11. Exposure time-dependent thermal effects of radiofrequency ... - OUCI
and Ishii, K. (2015): The effects of radio-frequency electromagnetic fields ... Akiko Matsumoto, Tatsuya Ishitake, Hiroshi Masuda. https://doi.org ...
https://doi.org/10.2131/jts.41.655 · Full text
12. (with Brigitte Steger) "Gender Matters" in Manga Girl Seeks Herbivore ...
... (Ishii-Kuntz 1992). In recent decades, however, the continuous drop in ... Kimmel 1996), Taga Futoshi has argued that the Japanese discipline of Men's ...
(with Brigitte Steger) "Gender Matters" in Manga Girl Seeks Herbivore Boys. Studies in Gender and Japanese Society from the University of Cambridge
